Journal: Frontiers in Pharmacology

Article Title: Blocking TRAIL-DR5 signaling pathway with soluble death receptor 5 fusion protein mitigates radiation-induced injury

doi: 10.3389/fphar.2023.1171293

Figure Lengend Snippet: sDR5-Fc blocked apoptosis in human small intestinal mucosal epithelial cells after irradiation. (A) Viability of human small intestinal mucosal epithelial cells at different radiation and administration doses. (B) Apoptosis percentage in human small intestinal mucosal epithelial cells at 24, 48 and 72 h after irradiation with or without 50 μg/mL sDR5-Fc. Quantitative data are shown as means ± SEM. * p ≤ 0.05, ** p ≤ 0.01, and *** p ≤ 0.001, as determined using one-way ANOVA and two-way ANOVA.

Article Snippet: Human small intestinal mucosal epithelial cells (CP-H039, Procell, China) were cultured in a complete medium for human small intestinal mucosal epithelial cells (CM-H039, Procell, China) and Intestinal Epithelioid Cell line No. 6 (IEC-6 cells) were cultured in Dulbecco’s Modified Eagle Medium (812619, gibco, United States) with 10% fetal bovine serum (FBS) (SV30208.02, cytiva HyClone, United States)and 1% Penicillin Streptomycin (15140-122, gibco, United States) and at 37°C under 5% CO 2 at atmosphere.

Techniques: Irradiation

Journal: International Journal of Molecular and Cellular Medicine

Article Title: The Expression Pattern and Clinicopathological Importance of Hsa_circ_000425 in Colorectal Cancer

doi: 10.22088/IJMCM.BUMS.9.4.266

Figure Lengend Snippet: Expression levels of hsa-circ_000425 in different colorectal cancer cell lines. The expression levels of hsa-circ_000425 in HCT8, SW480, SW620, and Lovo as cancerous cell lines, and normal colon epithelial cell line (NCM460) were evaluated .A significant down regulation of hsa-circ_000425 was observed in Sw620 (P < 0.01), Sw480 (P < 0.01), and Lovo (P < 0.001) cell lines. All values are given as mean ± SD of three independent experiments

Article Snippet: Cell culture Four CRC cell lines (SW480, SW620, HCT8, and Lovo) and NCM460 as normal human colon epithelial cell were purchased from the Cell Bank of Pasteur Institute (Tehran, IRAN).

Techniques: Expressing

Journal: European Journal of Nutrition

Article Title: Different infant formulas can activate toll-like receptor 9 in vitro and inhibit interleukin 6 in human primary intestinal epithelial cells

doi: 10.1007/s00394-024-03507-7

Figure Lengend Snippet: Expression and activity of TLR4 and TLR9 in human primary intestinal epithelial cells. a Western blot analysis of TLR4 and TLR9 in P-IECs, 0 h (basal) and 24 h after stimulation with TLR4 (LPS) or TLR9 agonist (ODN2006). GAPDH was used as reference protein. b Quantitative RT-PCR showing the levels of TLR4 and TLR9 in P-IECs 0 h, 2 h, and 6 h after stimulation with TLR4 (LPS) or TLR9 agonist (ODN2006). GAPDH was used as reference gene. n = 7/group. c, d HEK-Dual cells were stimulated with TLR9 agonist (ODN2006) for 2 h, 6 h, 11 h, and 24 h and the ( c ) optical density (OD 630 ) or ( d ) relative light units (RLUs) were compared with non-treated cells (basal). n = 4/group. e, f HEK-Dual cells were treated with a TLR9 agonist (A, ODN2006) or with inhibitor (INH-18) and agonist (I + A) in two different concentrations, 0.1 µg/ml ( e ) and 1.0 µg/ml ( f ), and were compared to non-treated cells. Luminescence signal was measured with a TECAN luminescence reader. n = 4/group. g MTT assay showing cell viability in different dilutions of the control formula, compared to control (Co) cells incubated with medium. n = 8/group. Data were analyzed by two-way ANOVA ( b–d ) or one-way ANOVA ( g ) followed by Newman-Keuls post hoc test or Student’s t -test ( e, f ) and are represented as mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001

Article Snippet: Adult human primary intestinal epithelial cells (P-IECs) were purchased from PELOBiotech GmbH (Martinsried, Germany).

Techniques: Expressing, Activity Assay, Western Blot, Quantitative RT-PCR, MTT Assay, Control, Incubation

Journal: European Journal of Nutrition

Article Title: Different infant formulas can activate toll-like receptor 9 in vitro and inhibit interleukin 6 in human primary intestinal epithelial cells

doi: 10.1007/s00394-024-03507-7

Figure Lengend Snippet: IL6 levels were decreased through various infant formulas in human primary intestinal epithelial cells; in contrast IL6 levels were increased due to TLR9 inhibition. The following phosphorylation stages and proteins were investigated by Western blot analysis in human P-IECs: TLR9, TLR4, pMAPK1/2 and MAPK1/2, pMAPK8/9 and MAPK8/9, pp38 and p38, pIRAK4 and IRAK4, IL6, NFKB, pIKB and IKB. HEK-Dual cells were cultivated in medium, PF, CF, and PHF in a 1/25 dilution, with TLR9 and TLR4 inhibitor (0.1 µg/ml) and non-treated HEK-Dual cells served as reference. In addition, the cells were incubated with L. fermentum CECT5716 (LC) (10 10 CFUs) for 30 min. GAPDH was used as reference protein

Article Snippet: Adult human primary intestinal epithelial cells (P-IECs) were purchased from PELOBiotech GmbH (Martinsried, Germany).

Techniques: Inhibition, Western Blot, Incubation

Journal: Oncotarget

Article Title: Hypermethylation of NDN promotes cell proliferation by activating the Wnt signaling pathway in colorectal cancer

doi: 10.18632/oncotarget.17580

Figure Lengend Snippet: (A) Immunostaining of NDN protein in CRC tissue samples and normal colorectal tissues. (B) Expression analyses of NDN protein in 12 surgical CRC tissues and the paired normal intestine epithelial samples using Western blot. β-tubulin was used as a loading control. (C) qRT-PCR analysis of NDN expression in 21 paired colorectal cancer tissues; NDN was quantified relative to the matched adjacent no tumor tissues. Error bars represent means ± SD calculated from three parallel experiments. (D) Expression analyses of NDN protein and mRNA in the normal intestinal epithelial cell FHC and five CRC cell lines through Western blot and qRT-PCR. Each bar represents the mean ± SD of three parallel replicates. (E) Influence of NDN expression on overall survival through Kaplan-Meier analysis in 84 CRC patients.

Article Snippet: Human normal intestinal epithelial cell FHC and five CRC cell lines SW480, HT29, HCT116, LS174T, and RKO were obtained from the The Global Bioresource Center (ATCC, USA).

Techniques: Immunostaining, Expressing, Western Blot, Control, Quantitative RT-PCR